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Updated: Aug 8, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The genetic diversity of mtDNA D-loop and the origin of Chinese goats
Ruo-Yu Liu1, Gong-She Yang, Chu-Zhao Lei
1College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Abstract:
The complete sequences of mitochondrial DNA D-loop of 128 individuals in nine Chinese goat (Capra hircu) breeds were analyzed by DNA sequencing technology. The results show that the length of mtDNA D-loop in Chinese goats is 1,212-1,213 bp. There are 102 polymorphic sites, accounting for 8.42% of 1,212 bp sequence. Ninety-two mtDNA haplotypes were determined. The haplotype diversity and nucleotide diversity are 0.9333-1.0000 and 0.7062%-1.8265%, respectively. The results indicate that the genetic diversity of Chinese goats is very abundant. The NJ tree indicates that Chinese goats have two types of maternal origins from lineage A and lineage B. The possibility of lineage B originating from China is also discussed.
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