[Determnination of betaine in Fufang Guilu granule by HPLC]
Ming-Wang Xu1, Xiu-Zhi Yuan, Yan-Weng Liu
1Affiliated Liyuan Hospital, Mongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, China. ligenluoxi@yahoo.com.cn
Objective:
To describe a HPLC method for assessing betaine in Fufang Guilu granule.
Method:
The content of betainephenaxcyl bromide in Fufang Guilu granule was determined by HPLC. The analytical column was a shim-pack CLC-ODS (6.0 mm x 150 mm) filling a 5 microm stationary phase; The mobile phase consisted of acetonitrile-water(35:65) with 0.1 mol x L(-1) NaClO4; The flow-rate was 1 mL x min (-1); The detector was set at 254 nm.
Result:
The calibration curve was linear over the range of 0.09-0.585 microg (r = 0.9997). The average recovery of the method was 98.4%, RSD 2.5% (n = 5).
Conclusion:
The results showed that this method was reliable and accurate, and can be used for quality control of Fufang Guilu granule.
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