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Studies on preimplantation development of buffalo embryos
C Karaivanov1, K Vlahov, M Petrov
1Bulgarian Academy of Sciences, Institute of Biology and Immunology of Reproduction and Development of Organisms, 73 Lenin Avenue, Sofia 1113 Bulgaria.
Theriogenology
|November 1, 1987
Summary
Superovulation induction using pregnant mare serum gonadotrophin (PMSG) or follicle stimulating hormone (FSH) effectively produced buffalo embryos. Embryo recovery rates varied based on flushing methods and timing post-estrus.
Area of Science:
- Animal Reproduction and Biotechnology
- Embryo Technology in Livestock
Background:
- Superovulation is crucial for enhancing reproductive efficiency in buffalo breeds.
- Hormonal treatments are commonly employed to stimulate multiple ovulations in donor animals.
Purpose of the Study:
- To evaluate the efficacy of superovulation protocols for embryo production in Murrah and crossbred buffaloes.
- To determine optimal timing and methods for embryo recovery in buffaloes.
Main Methods:
- 71 buffalo-cows received superovulation treatment with PMSG or FSH combined with PGF-2 alpha.
- Estrus detection followed by natural or artificial insemination.
- Nonsurgical uterine flushing or surgical flushing of reproductive tracts at various time points post-estrus.
Main Results:
- Embryo recovery yielded seven late morulae and eight hatched blastocysts between 114-116 hours post-estrus via nonsurgical flushing.
- All 40 embryos recovered after 117 hours were hatched blastocysts.
- Oviductal flushing yielded eggs earlier (74-100h), while combined oviductal and uterine flushing was more effective later (102-108h).
Conclusions:
- Superovulation protocols are effective for buffalo embryo production.
- Timing of embryo collection significantly influences the developmental stage and location of recovered embryos.