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Updated: Aug 8, 2026
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
Published on: January 20, 2018
Gene transfer in common carp (Cyprinus carpio L.) by microinjection into the germinal disc
O Hernández1, F O Castro, A Aguilar
1Mammalian Cell Genetics Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana, Cuba.
Abstract:
We report here on the microinjection of the human growth hormone gene (hGH) into the germinal disc of common carp one-cell embryos. For microinjection, a linear 4.1 kb EcoRi-DNA fragment containing the mouse metallothionein-I promoter (mMT-I), fused to a structural gene coding for the hGH, was used. Of the 14 injected embryos, 9 developed to an age of 60 d. Of these nine fish, two were found to be transgenic and had integrated two to four copies per cell of the hGH construct.
