Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Receptor-mediated immunomodulation by corticotropin-releasing factor.

T Audhya1, R Jain, C S Hollander

  • 1Department of Medicine, New York University Medical Center, NY 10016.

Cellular Immunology
|April 15, 1991
PubMed
Summary

Corticotropin-releasing factor (CRF) binds to immune cells, suppressing their function. This study confirms CRF

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced uptake and siRNA-mediated knockdown of a biologically relevant gene using cyclodextrin polyrotaxane.

Journal of materials chemistry. B·2020
Same author

Is a 'healthy diet' and a 'calcium-rich diet' the same thing? Qualitative study examining perceptions of a calcium-rich diet in individuals who have received bone health education.

Journal of human nutrition and dietetics : the official journal of the British Dietetic Association·2020
Same author

Mechanistic insights into controlled depolymerization of Chitosan using H-Mordenite.

Carbohydrate polymers·2020
Same author

Multiple simultaneous fractures are associated with higher all-cause mortality: results from a province-wide fracture liaison service.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2019
Same author

Simvastatin downregulates adipogenesis in 3T3-L1 preadipocytes and orbital fibroblasts from Graves' ophthalmopathy patients.

Endocrine connections·2019
Same author

Point-of-care urine lipoarabinomannan antigen detection for diagnosis of tuberculosis in children.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2019

Area of Science:

  • Immunology
  • Neuroendocrinology

Background:

  • Corticotropin-releasing factor (CRF) is implicated in stress responses and immune modulation.
  • Previous studies suggest CRF influences immune function, but specific cellular targets were unclear.

Purpose of the Study:

  • To investigate the direct binding of CRF to human immune cells.
  • To elucidate the functional consequences of CRF binding on immune cell activity.
  • To confirm the role of CRF in immunomodulation.

Main Methods:

  • CRF binding assays on human monocyte-macrophages and T-lymphocytes.
  • Measurement of intracellular cyclic AMP (cAMP) levels following CRF exposure.
  • Assessment of rat splenocyte proliferation in response to interleukin-2 (IL-2) with and without CRF.
  • Use of CRF antagonists to block CRF effects.

Main Results:

  • Specific binding sites for CRF were identified on human monocyte-macrophages and T-helper lymphocytes.
  • CRF binding led to increased intracellular cAMP concentrations in these cells.
  • CRF inhibited IL-2-induced rat splenocyte proliferation, an effect reversed by CRF antagonists.
  • CRF did not bind to T-suppressor or B lymphocytes.

Conclusions:

  • CRF possesses specific receptors on key immunocytes, including monocyte-macrophages and T-helper lymphocytes.
  • CRF binding modulates immune cell function, evidenced by cAMP production and suppressed proliferation.
  • These findings strengthen the evidence for CRF's significant role in neuroendocrine-immune interactions and immunomodulation.

Related Experiment Videos