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Updated: Aug 8, 2026

RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
Published on: June 4, 2016
Control of Listeria monocytogenes virulence by 5'-untranslated RNA
Edmund Loh1, Jonas Gripenland, Jörgen Johansson
1Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden.
Abstract:
The Gram-positive bacterium Listeria monocytogenes uses a wide range of virulence factors for its pathogenesis. Expression of five of these factors has previously been shown to be subjected to post-transcriptional regulation as a result of their long 5'-untranslated region (5'-UTR). We have investigated the presence of 5'-UTRs among the other known virulence genes and genes that encode putatively virulence-associated surface proteins. Our results strongly suggest that L. monocytogenes controls many of its virulence genes by a mechanism that involves the 5'-UTR. These findings further emphasize the importance of post-transcriptional control for L. monocytogenes virulence.
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