Carbonic anhydrase activators: activation of isozyme XIII with amino acids and amines
Seppo Parkkila1, Daniela Vullo, Luca Puccetti
1Institute of Medical Technology, University of Tampere, Tampere University Hospital, Biokatu 6, 33520 Tampere, Finland.
Abstract:
The first activation study of isoform XIII of carbonic anhydrase (CA, EC 4.2.1.1) is reported. A series of amino acids and amines incorporating protonatable moieties of the primary/heterocyclic amine type were included in the study. As for CA I and II, CA XIII activators enhance kcat and show no effect on KM, for the physiologic reaction catalyzed by this isoform. Excellent CA XIII activating properties were shown by D-amino acids (His, Phe, DOPA, and Trp), serotonin, and 4-(2-aminoethyl)-morpholine, whereas the corresponding L-amino acids, dopamine, histamine, and 1-(2-aminoethyl)-piperazine, were weaker activators.
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