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Updated: Jul 22, 2026

The Preparation of Electrohydrodynamic Bridges from Polar Dielectric Liquids
Published on: September 30, 2014
Thermodynamic analysis of autonomous parallel beta-sheet formation in water
John D Fisk1, Margaret A Schmitt, Samuel H Gellman
1Graduate Program in Biophysics and Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706, USA.
Abstract:
We report the first thermodynamic analysis of parallel beta-sheet formation in a model system that folds in aqueous solution. NMR chemical shifts were used to determine beta-sheet population, and van't Hoff anaysis provided thermodynamic parameters. Our approach relies upon the d-prolyl-1,1-dimethyl-1,2-diaminoethane unit to promote parallel beta-sheet formation between attached peptide strands. The development of a macrocyclic reference molecule to provide chemical shift data for the fully folded state was crucial to the quantitative anaylsis.
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