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Improved strategy for mononuclear cell collection for donor lymphocyte infusions.
Beth Shaz1, Katayoon Goodarzi, Elizabeth Malynn
1Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA. bshaz@bidmc.harvard.edu
Transfusion
|June 1, 2006
Summary
A new protocol successfully tailored whole blood processing during leukapheresis to achieve targeted cellular doses, improving donor lymphocyte collection efficiency and reducing procedure time.
Area of Science:
- Hematology
- Immunology
- Transfusion Medicine
Background:
- Donor management for lymphocyte collections requires optimization.
- Standard protocols may not consistently achieve desired cellular doses.
Purpose of the Study:
- To establish a protocol for tailoring whole blood volume processed during leukapheresis.
- To improve donor lymphocyte collection efficiency and safety.
Main Methods:
- Retrospective review of donor and product records (25 donors, 54 collections).
- Established correlation between total blood volume (TBV) processed and CD3+ T-cell yield.
- Applied correlation prospectively to guide TBV processing.
Main Results:
- Achieved 92% successful collections in the prospective group.
- Reduced TBV processed (12,598±4007 to 7942±5079 mL) and procedure time (208±53 to 146±79 min).
- Increased procedures without adverse events (23% to 37%).
Conclusions:
- Correlation data between TBV and T-cell yield effectively predicts collection success.
- Protocol application enhances efficiency and safety of donor lymphocyte collection.