Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoassays with direct mass spectrometric detection.

Alexis Nazabal1, Ryan J Wenzel, Renato Zenobi

  • 1Department of Chemistry and Applied Biosciences, ETH Zürich, CH-8093 Zürich, Switzerland.

Analytical Chemistry
|June 2, 2006
PubMed
Summary

A novel mass spectrometry method enables rapid, sensitive detection of protein-protein interactions. This high-throughput technique enhances drug discovery and diagnostics by analyzing antigen-antibody complexes with unprecedented speed and accuracy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasmonic Nanocavity-Induced Degradation Pathway of Boronic Acid Biosensing Interfaces Revealed by <i>In Situ</i> Tip-Enhanced Raman Spectroscopy.

ACS nano·2026
Same author

Noninvasive Analysis of Skin Emanations during Cupping Therapy by Thin-Film Solid-Phase Microextraction and Dielectric Barrier Discharge Ionization Mass Spectrometry.

Analytical chemistry·2026
Same author

Tracking Protein Misfolding and Oligomerization: A Temperature-Controlled Ion Mobility-Mass Spectrometry Approach.

Analytical chemistry·2026
Same author

Integrating Functional Response and Target Binding for Mechanism-Centered Drug Screening by High-Mass MALDI-MS.

ACS central science·2026
Same author

Engineered Strain in 2D Materials by Direct Growth on Deterministically Patterned Grayscale Topographies.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Nanoscale Chemical Analysis of Heterogeneous Catalysts Using Tip-Enhanced Raman Spectroscopy.

Chemical reviews·2026

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Immunology

Background:

  • Protein-protein interactions are vital for drug discovery and diagnostics.
  • Current mass spectrometry methods struggle with routine analysis of protein complexes.
  • A need exists for faster, more sensitive protein interaction detection techniques.

Purpose of the Study:

  • To present a new high-throughput strategy for analyzing protein interactions.
  • To demonstrate the application of this method in immunochemical analyses.
  • To overcome limitations of conventional methods for protein complex analysis.

Main Methods:

  • Utilized novel detector technology and chemical cross-linking to stabilize protein complexes.
  • Employed matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) for direct readout.

Related Experiment Videos

  • Developed a method for analyzing antigen-antibody complexes in the 150-400 kDa range.

    • Main Results:

    • Achieved high detection sensitivity, quantifying antigen in femtomole quantities.
    • Demonstrated high specificity, enabling direct antigen detection in serum.
    • Exhibited high accuracy and speed, with assays completed in minutes.
    • Outperformed conventional methods by over two orders of magnitude.

    Conclusions:

    • The developed mass spectrometry approach offers a rapid, specific, and sensitive solution for protein interaction analysis.
    • This methodology significantly advances high-throughput screening for drug discovery and clinical diagnostics.
    • The technique provides a robust platform for immunochemical applications like epitope mapping and kinetic studies.