Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An extra dimension in nucleic acid sequence recognition.

Keith R Fox1, Tom Brown

  • 1School of Biological Sciences, University of Southampton, Southampton, UK.

Quarterly Reviews of Biophysics
|June 2, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Vertebrate Genomes Project Phase I: A global reference genome resource.

bioRxiv : the preprint server for biology·2026
Same author

5'-Palmitate Lipid and Internal LNA-Piperidyl Triesters Enhance the RNA Affinity and Activity of Splice-Switching Oligonucleotides.

Journal of the American Chemical Society·2026
Same author

Ion chromatography-ultra-high-resolution mass spectrometry reveals EZH2-driven reprogramming of nucleic acid and protein methylation.

Analytica chimica acta·2026
Same author

Real-World Treatment Patterns for Patients with High-Risk Biochemically Recurrent Nonmetastatic Castration‑Sensitive Prostate Cancer.

Advances in therapy·2026
Same author

Non-enzymatic conversion of RNA sequence information into DNA by squaramide ligation for accurate RNA quantification.

Communications chemistry·2026
Same author

The reference genome of the Asian Elephant (Elephas maximus): a foundation for conservation and genomic research.

BMC genomics·2026
Same journal

Lasing emission spectroscopy for bioanalytics and biomedicine.

Quarterly reviews of biophysics·2026
Same journal

Elementary processes and mechanisms of nanopore formation induced by antimicrobial peptides and other membrane-active peptides.

Quarterly reviews of biophysics·2026
Same journal

Biomineralization: Perspectives on control of crystal polymorphism, order-disorder and solvation states.

Quarterly reviews of biophysics·2026
Same journal

The pivotal roles of cellular biophysics and mechanobiology in the development of Human Organs-on-Chips.

Quarterly reviews of biophysics·2026
Same journal

Biophysics meets fungal biology: Characterising the fungal cell envelope and its interactions with drug-like molecules.

Quarterly reviews of biophysics·2026
Same journal

Energy landscapes in molecular biology: History, principles, and perspectives.

Quarterly reviews of biophysics·2026
See all related articles

Modified nucleosides in triple-helix-forming oligonucleotides (TFOs) enable recognition of mixed DNA sequences. This advances nucleic acid interactions for new applications beyond Watson-Crick base pairing.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Watson-Crick base pairing is a fundamental molecular recognition process in biology.
  • Existing methods limit the complexity of nucleic acid interactions.
  • A need exists for additional sequence recognition modes to expand applications.

Purpose of the Study:

  • To develop a novel nucleic acid sequence recognition method.
  • To enable recognition of mixed DNA sequences using triplex formation.
  • To explore new applications in molecular biology and beyond.

Main Methods:

  • Incorporation of modified nucleosides into triple-helix-forming oligonucleotides (TFOs).
  • Utilizing TFOs to recognize specific DNA sequences.
  • Assessing selectivity and affinity of triplex formation at neutral pH.

Related Experiment Videos

Main Results:

  • Modified nucleosides enable TFOs to recognize mixed DNA sequences with high selectivity.
  • High affinity binding of TFOs to target DNA was achieved at neutral pH.
  • Ongoing research focuses on improving TFO performance at high pH and kinetics.

Conclusions:

  • The triplex approach offers a new dimension to nucleic acid interactions.
  • Modified TFOs provide a versatile tool for DNA sequence recognition.
  • Further development of TFOs promises diverse applications in biotechnology and medicine.