Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Timing controllable electrofusion device for aqueous droplet-based microreactors.

Wei-Heong Tan1, Shoji Takeuchi

  • 1CIRMM/IIS, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. takeuchi@iis.u-tokyo.ac.jp

Lab on a Chip
|June 2, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same authorSame journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026
Same author

Cell-Based Multisensor Array for Vapor-Phase Detection of Cancer-Related Compounds in Human Urine.

ACS sensors·2026
Same author

Prevascularized grafts with spatially organized MSC spheroids to accelerate therapeutic angiogenesis in ischemic disease.

Angiogenesis·2026
Same author

Longitudinal tracking of dyadic body temperature synchrony in social pairs across species.

Scientific reports·2026
Same author

Dynamic in vitro platform for mechanical profiling of human pulmonary aciniform organoids via intraluminal access.

Biomaterials·2026
Same author

Cell-based biohybrid sensing of a volatile aggregation pheromone component associated with the invasive red palm weevil.

Biosensors & bioelectronics·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026
Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026
See all related articles

This study introduces an electrofusion device for precise droplet fusion, enabling accurate reaction timing and minimizing surface absorption issues. The technology facilitates controlled chemical and biological experiments with pico-litre droplet manipulation.

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Microfluidics

Background:

  • Controlling droplet fusion is crucial for precise reaction initiation in microfluidic systems.
  • Surface absorption can interfere with reactions when reactants contact channel walls.

Purpose of the Study:

  • To develop an electrofusion device for controlled droplet merging.
  • To enable accurate timing of chemical and biological reactions.
  • To minimize reactant loss due to surface absorption.

Main Methods:

  • An electrofusion device was designed to apply electric fields for droplet fusion.
  • Enzymatic reactions were initiated by fusing droplets containing beta-galactosidase and fluorescein di-beta-D-galactopyranoside (FDG).
  • Fluorescence microscopy was used for reaction observation.

Related Experiment Videos

Main Results:

  • The device precisely controlled the moment of droplet fusion.
  • Accurate determination of reaction initiation was achieved.
  • Surface absorption issues were eliminated by minimizing reactant contact with channel walls.
  • Sequential fusion of pico-litre droplets was successfully demonstrated.

Conclusions:

  • The electrofusion device offers a robust method for controlled microdroplet fusion.
  • This technology enhances the accuracy and reliability of microfluidic reaction studies.
  • The device facilitates easy fabrication and continuous observation of initiated reactions.