Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nucleotide Excision Repair

Nucleotide Excision Repair

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

Base Excision Repair

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

Nucleotide Excision Repair

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Osmotic signaling governs sunscreen biosynthesis to safeguard desert cyanobacteria against desiccation.

mLife·2026

Same author

Phase separation of the redox sensor RCD1 mediates differential ROS signals to regulate plant growth and stress responses.

Molecular plant·2026

Same author

Engineering seed-specific gossypol-free cotton for human-safe consumption by harnessing the dominant-negative effect of the Gl<sub>2</sub><sup>e</sup> mutation.

Plant communications·2025

Same author

The splicing factor U2AF65B regulates cytosine methylation through interacting with DEFECTIVE IN MERISTEM SILENCING 3 in Arabidopsis.

The New phytologist·2025

Same author

Genetic manipulation of the genes for clonal seeds results in sterility in cotton.

BMC plant biology·2024

Same author

Herbicide-resistant 4-hydroxyphenylpyruvate dioxygenase variants identified via directed evolution.

Journal of experimental botany·2024

Search research articles

Related Experiment Video

Updated: Jul 8, 2026

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Published on: February 18, 2009

RNA extraction.

Huazhong Shi¹, Ray Bressan

¹Texas Tech University, Department of Chemistry and Biochemistry, Lubbock, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 3, 2006

Summary

This study details two plant RNA extraction methods for Arabidopsis. The small-scale method is versatile for most molecular biology applications, while the large-scale method is specific for Northern hybridization.

Area of Science:

Molecular Biology
Plant Science

Background:

High-quality RNA extraction is crucial for plant molecular biology experiments.
Techniques like reverse transcription-polymerase chain reaction and microarray analysis depend on reliable RNA isolation.

Purpose of the Study:

To describe two distinct RNA extraction methods for Arabidopsis.
To differentiate the applications of small-scale versus large-scale RNA extraction.

Main Methods:

Detailed protocol for a small-scale RNA extraction from Arabidopsis.
Detailed protocol for a large-scale RNA extraction from Arabidopsis.

Main Results:

The small-scale RNA extraction method yields high-quality RNA suitable for various downstream applications.

More Related Videos

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

Related Experiment Videos

Last Updated: Jul 8, 2026

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Published on: February 18, 2009

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

The large-scale RNA extraction method is optimized specifically for Northern hybridization.

Conclusions:

Both small-scale and large-scale RNA extraction methods are effective for Arabidopsis.
Method selection depends on the specific downstream application, with small-scale being broadly applicable and large-scale specialized for Northern hybridization.