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Updated: Aug 8, 2026
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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Published on: June 16, 2021
Oocyte expression with injection of purified T7 RNA polymerase
Xavier Altafaj1, Nathalie Joux, Michel Ronjat
1Département Réponse et Dynamique Cellulaire, Inserm U607, Grenoble, France.
Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2006
Abstract:
The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
