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Updated: Aug 8, 2026
Optimized PCR-based Detection of Mycoplasma
Published on: June 20, 2011
[Sequential analysis of detection Mycoplasma in different positions of female genital tract]
Bei Wang1, Hong Yu, Jin-shui Xu
1Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China.
Objective:
To discuss the main parasitic position of Ureaplasma urealyticum (Uu) and Mycoplasma hominis (Mh) in female genital tract.
Methods:
Using the standard aseptic cotton swab to collect secretion in vaginal fornix and orificium internum uteri, to culture Uu and Mh in Mycoplasma ID medium of France Bio-Merieux Co. According to double-direction quality reaction sequential test design, detection results of different position were analyzed.
Results:
Total positive and > or = 10(4) ccu/ml positive of Uu in vaginal fornix were significantly higher than that in orificium internum uteri. Total positive of Mh in vaginal fornix was significantly higher than that in orificium internum uteri as well.
Conclusion:
In order to raise the detectable rate of Mycoplasma, we suggested that the secretion in vaginal fornix position be collected.
