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Published on: January 11, 2019
[Immunoglobulin expression in colon cancer cell line HT-29 and its biological activities]
Yu-qing Deng1, Jie Zheng, Guo-hui Li
1Peking University Center for Human Disease Genomics, Beijing 100083, China.
Objective:
To determine the expression of immunoglobulins in HT-29 cells (an established colon cancer cell line, and explore their effect on the biological activities of the cancer cells.)
Methods:
The transcripts of variable regions of immunoglobulin heavy chains in HT-29 cells were detected by RT-PCR. Antisense CDR3 (specific to HT-29)-pIRES 1 neo vector was constructed, then transfected into HT-29 cells by electroporation. Programmed cell death and growth inhibition of HT-29 cells were detected by FCM and MTT, respectively.
Results:
The transcripts of Ig heavy chain (V(H) CDR3 region) were expressed in HT-29 cells. Moreover, they showed a monoclonal characteristic after being sequenced. After transfection of the antisense vector of CDR3 (specific to HT-29)-pIRES 1 neo, expression level of Ig in HT-29 cells was significantly decreased, and growth inhibition (P < 0.05) and apoptosis (P < 0.01) were induced.
Conclusion:
These results suggest that tumor derived Ig could promote the survival and growth of tumor cells.
