Related Experiment Video
Updated: Aug 8, 2026
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
Published on: December 3, 2011
Prevalence and identity of Cryptosporidium spp. in pig slurry
Lihua Xiao1, John E Moore, Ukeme Ukoh
1Division of Parasitic Diseases, Centers for Disease Control and Prevention, Mail Stop F12, 4770 Buford Highway, Atlanta, GA 30341, USA. lxiao@cdc.gov
Abstract:
Cryptosporidium spp. were detected in 25 of 56 pig slurry samples from 33 Irish farms by PCR and DNA sequencing. The organisms detected included C. suis, Cryptosporidium pig genotype II, and C. muris. We concluded that Cryptosporidium oocysts can persist in treated slurry and potentially contaminate surface water through improper discharge or uncontrolled runoff.
Insights
Cryptosporidium parasites were found in Irish pig slurry, indicating oocysts can survive treatment. This poses a risk of surface water contamination from slurry discharge or runoff.
Area of Science:
- Veterinary Parasitology
- Environmental Microbiology
- Molecular Diagnostics
Background:
- Cryptosporidium is an important enteric protozoan parasite.
- Pig slurry is a potential reservoir for transmitting Cryptosporidium to the environment.
Purpose of the Study:
- To investigate the prevalence and species of Cryptosporidium in Irish pig slurry.
- To assess the potential for environmental contamination from treated pig slurry.
Main Methods:
- Polymerase Chain Reaction (PCR) and DNA sequencing were used to detect Cryptosporidium DNA.
- Samples were collected from 56 pig slurry batches across 33 Irish farms.
Main Results:
- Cryptosporidium was detected in 25 out of 56 (44.6%) slurry samples.
- Identified species included Cryptosporidium suis, Cryptosporidium pig genotype II, and Cryptosporidium muris.
Conclusions:
- Cryptosporidium oocysts can persist in treated pig slurry.
- Improper slurry discharge or runoff can lead to surface water contamination.
