Related Experiment Videos
Lipid mediator informatics and proteomics in inflammation resolution.
Yan Lu1, Song Hong, Katherine Gotlinger
1Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. ylu@zeus.bwh.harvard.edu
Thescientificworldjournal
|June 6, 2006
Summary
Lipid mediator informatics combined with proteomics identifies novel biomarkers for inflammation and disease. This approach uses advanced analytical techniques to uncover bioactive lipid mediators and their roles in health and illness.
Area of Science:
- Biochemistry and Systems Biology
- Proteomics and Bioinformatics
Background:
- Lipid mediator informatics is crucial for identifying bioactive lipid mediators (LMs) and their pathways.
- LM informatics and proteomics are powerful tools for discovering biomarkers in health and disease.
- Understanding LM roles in pathophysiology requires integrating system biology analysis.
Purpose of the Study:
- To review initial steps in elucidating relationships among biomolecular classes.
- To provide an appreciation of LM roles and activities in disease pathophysiology.
- To present detailed laboratory protocols for LM informatics and proteomics.
Main Methods:
- Liquid chromatography-ultraviolet-tandem mass spectrometry (LC-UV-MS/MS)
- Gas chromatography-mass spectrometry (GC-MS)
- Enzyme-linked immunosorbent assay (ELISA)
- Computer-based automated systems with databases and searching algorithms
- 2-dimensional gel electrophoresis and LC-nanospray-MS/MS peptide mapping
Main Results:
- Identification of novel mediators in inflammation resolution.
- Profiling temporal and spatial production of lipid mediators.
- Elucidation of relationships among biomolecular classes involved in disease.
Conclusions:
- LM informatics and proteomics are essential for advancing our understanding of inflammation and disease.
- Automated systems with databases and algorithms are critical for accurate LM analysis.
- These methods enable the identification of key lipid mediators, such as eicosanoids, resolvins, and neuroprotectins.