A simple and practical agglutination assay for human leucocyte antigen-B27 typing

Area of Science:

• Immunogenetics
• Clinical diagnostics

Background:

• Human leucocyte antigen (HLA) B27 is a frequently typed antigen associated with various diseases.
• Accurate and efficient HLA-B27 typing is crucial for disease association studies and clinical management.

Purpose of the Study:

• To develop and evaluate a simple and rapid particle agglutination assay (PaGIA) for human leucocyte antigen (HLA) B27 typing.

Main Methods:

• Superparamagnetic particles coated with anti-HLA-B27 monoclonal antibodies were used.
• The assay was tested on whole-blood samples from healthy donors (n=194) and patients (n=51) previously typed by flow cytometry.

Main Results:

• The particle agglutination assay demonstrated clear positive reactions with HLA-B27 positive blood samples.
• The assay showed high concordance with flow cytometry, with only one indeterminate result observed.

Conclusions:

• The developed HLA-B27 PaGIA is a suitable method for rapid and accurate HLA-B27 typing.
• The assay is simple, easy to perform, and adaptable for routine laboratory use.