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A tagging-via-substrate technology for genome-wide detection and identification of farnesylated proteins

Sung Chan Kim1, Yoonjung Kho, Deb Barma

  • 1Department of Biochemistry, UT Southwestern Medical Center at Dallas, Dallas, Texas, USA.

Methods in Enzymology
|June 8, 2006
PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...