Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoassay techniques.

Michael J Wheeler1

  • 1Department of Chemical Pathology, St. Thomas' Hospital, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|June 10, 2006
PubMed
Summary
This summary is machine-generated.

Immunoassay revolutionized hormone measurement, offering accessible methods for producing reagents and performing assays for various hormones. This guide provides practical techniques for reliable immunoassay development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Atmospheric aging of tire rubber antioxidants forms complex mixtures that trigger inflammation in human macrophages.

Environment international·2026
Same author

Mentally Active Versus Passive Sedentary Behavior and Risk of Dementia: 19-Year Cohort Study.

American journal of preventive medicine·2026
Same author

Reply to the letter : To consider the exercise density in the dose-response relationship: the idea is promising, the operationalization tricky!

European journal of applied physiology·2025
Same author

Interrupting prolonged sitting reduces postprandial GIP but not GLP-1 responses in type 2 diabetes.

Diabetes, obesity & metabolism·2025
Same author

Beyond FITT: addressing density in understanding the dose-response relationships of physical activity with health-an example based on brain health.

European journal of applied physiology·2025
Same author

Multiphase OH Oxidation of Bisphenols: Chemical Transformation and Persistence in the Environment.

Environmental science & technology·2025
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Area of Science:

  • Biochemistry
  • Immunology
  • Analytical Chemistry

Background:

  • Immunoassay represents a significant advancement in hormone measurement technology.
  • Commercial availability of reagents and kits is limited for novel or less common hormones.

Purpose of the Study:

  • To explain the fundamental principles of immunoassay reactions.
  • To provide practical methods for developing immunoassays and immunometric assays.
  • To guide the production of reagents for both antigenic and hapten hormones.

Main Methods:

  • Detailed explanation of the basic immunoassay reaction.
  • Simple, validated methods for performing immunoassays and immunometric assays.
  • Procedures for preparing labeled hormones and various separation techniques.

Related Experiment Videos

Main Results:

  • The described methods facilitate the creation of usable immunoassays.
  • Techniques are applicable to a broad spectrum of hormone assays.
  • Previously validated methods ensure reliability and efficiency.

Conclusions:

  • This chapter equips researchers with the knowledge to produce effective immunoassays.
  • The provided methods enable the development of assays for esoteric and new hormones.
  • Established techniques ensure the successful and timely production of hormone immunoassays.