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Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
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Expression of tumor-associated antigen RCAS1 and its possible involvement in immune evasion in oral squamous cell

T Toyoshima1, S Nakamura, W Kumamaru

  • 1Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
|June 10, 2006
PubMed
Abstract

Insights

Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) expression in oral squamous cell carcinoma (SCC) correlates with increased tumor-infiltrating lymphocyte (TIL) apoptosis, suggesting a role in immune evasion.

Area of Science:

  • Oncology
  • Immunology

Background:

  • Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) induces apoptosis in receptor-positive cells.
  • RCAS1 is investigated in oral squamous cell carcinoma (SCC) and its impact on tumor-infiltrating lymphocytes (TILs).

Purpose of the Study:

  • To investigate RCAS1 expression in oral SCC.
  • To determine the association between RCAS1 expression and TIL apoptosis in oral SCC.

Main Methods:

  • Immunohistochemistry used to examine RCAS1 expression in 130 oral SCC patients.
  • Terminal Deoxynucleotidyltransferase-mediated dUTP Nick End Labeling (TUNEL) staining used to assess TIL apoptosis.

Main Results:

  • RCAS1 detected in tumor cells (cytoplasm and membrane) in 31.5% of cases.
  • RCAS1 expression at the invasive front observed in 16.9% of cases.
  • Significantly higher percentage of TUNEL-positive TILs in RCAS1-positive SCC cases (P < 0.0001).

Conclusions:

  • RCAS1 is expressed on oral SCC cells.
  • RCAS1 may facilitate tumor escape from the host immune system.
  • RCAS1 induces apoptosis of TILs, contributing to immune evasion.

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