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[Expression and effects of nuclear distribution C (NUDC) protein in nasopharyngeal carcinoma cell lines]
Yan-Qiu Chen1, Tian-Ying Li, Shen-Hong Qu
1Hospital of Otorhinolaryngology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510080, P. R. China. frankcyq2002@yahoo.com.cn
Background & Objective:
Nuclear migration is essential for growth, development, and cellular function of eukaryotes. Nuclear distribution C (NUDC) protein plays an important role in nuclear migration. This study was to detect the expression of NUDC protein in nasopharyngeal carcinoma (NPC) cell lines CNE-2 and HNE-2, and to investigate the effect of anti-NUDC antibody on the growth of both cell lines.
Methods:
Soluble fusion protein GST-NUDC was expressed in the cytoplasm of Escherichia coli, and its poly-antibody was prepared. The subcellular localization of NUDC protein in CNE-2 and HNE-2 cells was detected with indirect immunofluorescent staining, the expression of NUDC protein in CNE-2 and HNE-2 cells was detected by Western blot, the effect of anti-NUDC antibody on the growth of CNE-2 and HNE-2 cells was detected by MTT assay.
Results:
NUDC protein was expressed in both nasopharyngeal non-cancerous tissues and CNE-2 and HNE-2 cells, and its expression was higher in CNE-2 and HNE-2 cells than in nasopharyngeal non-cancerous tissues. NUDC protein in CNE-2 and HNE-2 cells was distributed mainly in the karyolemma of cytoplasm, and in nuclei asymmetrically. Anti-NUDC antibody inhibited the growth of CNE-2 and HNE-2 cells in a dose-dependent manner.
Conclusions:
NUDC protein is expressed highly in CNE-2 and HNE-2 cells. Anti-NUDC antibody could inhibit the growth of CNE-2 and HNE-2 cells.
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