Related Experiment Video
Updated: Feb 27, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Single-molecule approaches reveal the idiosyncrasies of RNA polymerases
Jordanka Zlatanova1, William T McAllister, Sergei Borukhov
1Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA. jordanka@uwyo.edu
Abstract:
Recently developed single-molecule techniques have provided new insights into the function of one of the most complex and highly regulated processes in the cell--the transcription of the DNA template into RNA. This review discusses methods and results from this emerging field, and it puts them in perspective of existing biochemical and structural data.
Related Concept Videos
Eukaryotic RNA Polymerases
All three eukaryotic RNAPs require specific transcription factors, of which the...
Eukaryotic RNA Polymerases
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Bacterial RNA Polymerase
Transcription Initiation
The promoters and enhancers and their accessory proteins allow tight regulation of...
Proofreading
Errors During Replication are Corrected by the DNA Polymerase...

