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Updated: Sep 5, 2026

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
Published on: July 20, 2016
Beyond the thiolate: Discovering natural variation in cytochrome P450 proximal ligation
Jessica A Gable1, Alec H Follmer2
1Department of Chemistry, University of California, Davis, Davis, CA 95616, USA.
Abstract:
In a recent issue of Nature Chemical Biology, Nguy et al.1 report the discovery of an array of cytochrome P450 enzymes that challenge the long-standing view that P450 hemes must be coordinated by a cysteine thiolate. Many of these "noncanonical" P450s instead use serine or selenocysteine ligands. The characterization of two selected variants revealed distinct structural and spectroscopic features.
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