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[Preparation of polyclonal antibody against microcystin-LR]
Jian-wu Sheng1, Miao He, Han-chang Shi
1ESPC State Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China.
Abstract:
Good quality polyclonal antibody against Microcystin-LR (MC-LR) was obtained from the New Zealand rabbit immunized with the homemade complete antigen BSA-MC-LR. Indirect ELISA shows the titer of this antibody is more than 1.5 x 10(5); Indirect competitive ELISA immobilized the coating antigen OVA-MC-LR was adapted to detect MCs, the calibration curve shows that the detection lower limit is 10 ng/L, and the range of quantitative detection was between 30 ng/L to 3 microg/L, with a good linearity, which will well potentially suit for sensitive analysis for MC-LR in drinking as well as surface water samples.
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