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IL-2-induced IL-13 production by allergen-specific human helper T cell clones
Tomomi Hashimoto1, Noriaki Kobayashi, Yuichiro Kajiyama
1Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Kanagawa, Japan.
Background:
Interleukin (IL)-5 and IL-13 are important cytokines in allergic diseases such as asthma and atopic dermatitis. We have reported that the production of IL-5 and IL-13 by mite-responsive T helper cells (Th) is controlled under similar signal requirements, but precise mechanisms are not yet well characterized.
Methods:
Allergen-specific Th clones were established from peripheral blood lymphocytes of atopic asthmatics, and cytokine synthesis in response to various stimuli was determined by specific ELISAs. IL-13 gene expression was enumerated by quantitative real-time PCR.
Results:
IL-13 production was clearly induced by IL-2. IL-13 mRNA expression was also induced.
Conclusion:
The IL-2 signal by itself causes IL-13 synthesis independent of T cell receptor stimulation.
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