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Updated: Aug 7, 2026
09:08
Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
Published on: May 31, 2012
A human protein atlas based on antibody proteomics
Anja Persson1, Sophia Hober, Mathias Uhlén
1Royal Institute of Technology, School of Biotechnology, AlbaNova University Center, SE-106 91 Stockholm, Sweden. anja@biotech.kth.se
Current Opinion in Molecular Therapeutics
|June 16, 2006
Summary
The Human Protein Atlas database offers extensive protein expression data for human tissues. Open access and rigorous validation ensure reliable protein and antibody data for research.
Area of Science:
- Biotechnology
- Genomics
- Proteomics
Background:
- The Human Protein Atlas is a vital resource for understanding human protein expression.
- It provides immunohistological data across numerous human tissues.
Purpose of the Study:
- To review the Human Protein Atlas database contents and project strategy.
- To emphasize the significance of open access for data validation and quality.
Main Methods:
- Utilizes protein epitope signature tags (PrEST) antigens for antibody production.
- Employs monospecific antibodies, tissue microarrays, and rigorous quality control.
- Presents protein expression profiles through immunohistological images.
Main Results:
- The atlas contains comprehensive protein expression profiles for human proteins.
- Detailed methodologies ensure high-quality antibody production and image generation.
- The project strategy prioritizes open access for broad data utility.
Conclusions:
- The Human Protein Atlas is a crucial, open-access resource for biomedical research.
- Its robust validation procedures guarantee data reliability.
- It integrates with other expression atlas initiatives, enhancing collaborative research potential.
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