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Updated: May 31, 2026

RhoC GTPase Activation Assay
Published on: August 22, 2010
PI3K and RAC signalling in leukocyte and cancer cell migration
Mark A Barber1, Heidi C E Welch
1Inositide Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, UK.
Abstract:
Leukocytes and amoeboid-stage cancer cells migrate in a similar manner. Key signalling molecules regulating leukocyte and cancer cell migration are phosphoinositide 3-kinase (PI3K) and the Rho family of small GTPases. While PI3K activity defines the leading edge of the cell, Rho family GTPases regulate the cytoskeletal remodelling during polarisation and migration. We review here briefly the roles of PI3K, of the Rho-family GTPase Rac, and of the guanine-nucleotide exchange factors that activate Rac, in leukocyte and cancer cell migration.
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