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[Cloning, expression and subcellular localization of a novel human gene-RNF122]
Li Wang1, Tai-ping Shi, Lan Wang
1Department of Immunology, Peking University School of Basic Medical Science Center for Human Disease Genomics, Peking University, Beijing 100083, China.
Objective:
To clone a functionally unknown gene RNF122 and analyze its expression pattern and subcellular localization.
Methods:
PCR was used to clone the novel gene-RNF122 from the mixed human tissue cDNA library. Bioinformatics analysis was used to identify structure characteristics of the gene. Northern blot was used to analyze its expression in normal tissues; RT-PCR was employed for its expression in cell lines and tumor tissues. By co-focal microscope, we identified its subcellular localization with organelle markers.
Results:
A novel human gene-RNF122 was cloned, which was proved to have been expressed in several normal and tumor tissues and many cell lines, and localized in ER and Golgi apparatus.
Conclusion:
RNF122 is a novel gene which encodes a protein that has a classic RING domain. It is widely expressed in several tissues and cell lines. The encoded protein is localized in ER and Golgi apparatus, which indicates that it may play a role in the process of protein degradation.
