Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Miniaturized separation techniques in glycomic investigations.

Yehia Mechref1, Milos V Novotny

  • 1National Center for Glycomics and Glycoproteomics, Department of Chemistry, Indiana University, 800 E Kirkwood Ave, Bloomington, IN 47405, United States.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|June 20, 2006
PubMed
Summary

High-sensitivity glycomic analysis is crucial for biomedical research and recombinant protein development. This review highlights advanced separation techniques like capillary electrophoresis (CE) and capillary electrochromatography (CEC) for sensitive glycomic profiling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cnidarian-algal partnerships structure bacterial communities during strobilation in <i>Cassiopea xamachana</i>.

ISME communications·2026
Same author

Metabolic Remodeling of the Parkinson's Disease Frontal Cortex Revealed by LC-MS/MS Metabolomics.

Biomolecules·2026
Same author

High-resolution LC-MS/MS analysis of brain N-glycans reveals composition-specific changes in Parkinson's disease.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same author

Qualitative Characterization of Neu5Gc-Containing N-Glycans in Commercial Cheddar Cheese Products Using Nano-UHPLC-ESI-MS/MS-A Pilot Study.

International journal of molecular sciences·2026
Same author

Direct Comparison of MRM-MS and PRM-MS Methods for Quantitative Ganglioside Analysis.

Journal of the American Society for Mass Spectrometry·2026
Same author

Parkinson's Disease-Associated Remodeling of Synaptic <i>N</i>-Glycans in the Human Prefrontal Cortex.

Journal of proteome research·2026

Area of Science:

  • Biomedical research
  • Analytical chemistry
  • Glycoscience

Background:

  • High-sensitivity glycomic analyses are increasingly important in biomedical and clinical research.
  • These analyses are also vital for the development of recombinant protein products.
  • Understanding glycan structures is key to biological function and disease mechanisms.

Purpose of the Study:

  • To review the evolution of separation techniques for high-sensitivity glycomic analysis.
  • To highlight advanced methodologies applicable to complex biological samples.
  • To demonstrate the potential of these techniques in glycomic profiling.

Main Methods:

  • Capillary liquid chromatography
  • Capillary electrophoresis (CE)

Related Experiment Videos

  • Capillary electrochromatography (CEC)

    • Main Results:

    • These separation techniques enable high-sensitivity glycomic analysis.
    • Demonstrated applicability to model glycoproteins.
    • Successful analysis of total glycomes from biological samples.

    Conclusions:

    • Advanced separation techniques like CE and CEC are pivotal for sensitive glycomic analysis.
    • These methods offer significant potential for both research and clinical applications.
    • Further development in this area will enhance our understanding of glycomic complexity.