Related Experiment Video
Updated: Aug 7, 2026
09:45
Detection of Copy Number Alterations Using Single Cell Sequencing
Published on: February 17, 2017
Invader assay for single-nucleotide polymorphism genotyping and gene copy number evaluation
Andrea Mast1, Monika de Arruda
1Third Wave Technologies Inc., Madison, WI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 21, 2006
Summary
The Invader assay offers sensitive DNA detection using signal amplification, not target amplification. This accurate method analyzes nucleic acid sequences for various genetic variations and gene expression.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- The Invader assay is a homogeneous, isothermal DNA probe-based method.
- It enables sensitive detection of nucleic acid sequences.
Purpose of the Study:
- To describe the Invader assay's capabilities for nucleic acid detection.
- To highlight its accuracy and specificity in various analyses.
Main Methods:
- Utilizes a homogeneous, isothermal DNA probe-based approach.
- Performs reactions directly on genomic DNA or total RNA.
- Can also use amplified products from polymerase chain reaction (PCR) or reverse transcriptase polymerase chain reaction (RT-PCR).
- Employs target-specific signal amplification for detection, avoiding target amplification.
Main Results:
- Achieves sensitive detection of nucleic acid sequences.
- Demonstrates high accuracy and specificity.
- Enables both qualitative and quantitative analysis.
- Suitable for detecting single-nucleotide changes, insertions/deletions, gene copy number, infectious agents, and gene expression.
Conclusions:
- The Invader assay is a versatile and precise tool for molecular diagnostics.
- Its signal amplification strategy offers a unique advantage for sensitive nucleic acid detection.
- Applicable across a broad range of genetic and infectious disease research.
