Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isotope-coded affinity tags for protein quantification.

Christopher M Colangelo1, Kenneth R Williams

  • 1W. M. Keck Foundation Biotechnology Resource Laboratory, Yale University, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 21, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multi-omic approach implicates novel protein dysregulation in post-traumatic stress disorder.

Genome medicine·2025
Same author

Closed-loop automated drug infusion regulator: A clinically translatable, closed-loop drug delivery system for personalized drug dosing.

Med (New York, N.Y.)·2024
Same author

Optimal conditions for carrying out trypsin digestions on complex proteomes: From bulk samples to single cells.

Journal of proteomics·2024
Same author

Differential Effects of Cocaine and Morphine on the Diurnal Regulation of the Mouse Nucleus Accumbens Proteome.

Journal of proteome research·2023
Same author

Differential Effects of Cocaine and Morphine on the Diurnal Regulation of the Mouse Nucleus Accumbens Proteome.

bioRxiv : the preprint server for biology·2023
Same author

Deciphering Spatial Protein-Protein Interactions in Brain Using Proximity Labeling.

Molecular & cellular proteomics : MCP·2022
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This study introduces an isotope-coded affinity tag technique for comparing protein levels in biological samples. This method enhances protein identification and quantitation in mass spectrometry experiments.

Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Comparing protein abundance across biological samples is crucial for understanding physiological states.
  • Existing methods face challenges in reducing sample complexity and increasing protein identification.
  • Mass spectrometry is a key technology for protein analysis.

Purpose of the Study:

  • To develop and apply a novel technique for quantitative proteomics.
  • To improve the efficiency and depth of protein identification in complex biological samples.
  • To enable accurate comparison of protein expression levels between different conditions.

Main Methods:

  • Utilizing isotope-coded affinity tags that selectively bind to cysteine residues.
  • Differential isotopic labeling of two biological samples (heavy and light).

Related Experiment Videos

  • Combining, separating via chromatography, and analyzing samples using tandem mass spectrometry.
  • Database searching of mass spectrometry data to identify proteins and quantify ratios.

    • Main Results:

    • Significant reduction in peptide mixture complexity.
    • Increased number of protein sequences identified per experiment.
    • Generation of a comprehensive list of hundreds of proteins with their heavy:light ratios.
    • Enabling relative quantitation of protein abundance.

    Conclusions:

    • The isotope-coded affinity tag technique is effective for quantitative proteomics.
    • This method significantly enhances protein identification and quantitation capabilities.
    • It provides a powerful tool for correlating protein expression changes with physiological states.