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Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
Published on: June 10, 2017
Genomic alterations in Hodgkin's lymphoma
Marc A Weniger1, Thomas F E Barth, Peter Möller
1Department of Pathology, University of Ulm, Ulm, Germany.
International Journal of Hematology
|June 22, 2006
Summary
Genomic analysis reveals distinct chromosomal aberrations in classic Hodgkin
Area of Science:
- Molecular genetics
- Cancer genomics
- Hematopathology
Background:
- Cytogenetic analysis of Hodgkin's lymphoma (HL) is challenging due to the low proportion of neoplastic cells.
- HL is increasingly recognized as two distinct entities: classic HL (cHL) and nodular lymphocyte predominant HL (NLPHL).
- Previous studies utilized morphology and immunohistochemistry to differentiate HL subtypes.
Purpose of the Study:
- To review genomic aberrations contributing to the development and presentation of cHL and NLPHL.
- To highlight molecular cytogenetic findings substantiating the subdivision of HL into distinct disease entities.
- To explore epigenetic mechanisms influencing gene expression in HL neoplastic cells.
Main Methods:
- Comparative genomic hybridization (CGH) analysis on microdissected cells and cell lines from cHL patients.
- Review of existing molecular cytogenetic investigations in HL.
- Examination of epigenetic mechanisms regulating gene expression in HL.
Main Results:
- Recurrent chromosomal aberrations, specifically gains of 2p13-p16 and 9p24, identified in cHL but not NLPHL.
- cHL cell lines exhibit heterogeneous karyotypes, indicating significant genomic instability.
- Genomic aberrations and epigenetic modifications are implicated in HL pathogenesis.
Conclusions:
- Molecular cytogenetics provides strong support for classifying HL into cHL and NLPHL.
- Specific genomic gains (2p13-p16, 9p24) are characteristic of cHL.
- Genomic instability and epigenetic alterations play crucial roles in HL development and clinical manifestation.
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