Microbial hydrolysis of acetylated nucleosides
Julieta Panero1, Jorge Trelles, Valeria Rodano
1Universidad Nacional de Quilmes, RS Peña 352 (1876), Bernal, Buenos Aires, Argentina.
Biotechnology Letters
|June 22, 2006
Summary
Microbial whole cells were used for the first time to enzymatically deacetylate nucleosides. Different microorganisms showed varied deacetylation profiles, yielding selective or mixed mono-acetylated products depending on the biocatalyst and substrate.
Area of Science:
- Biochemistry
- Enzymology
- Microbiology
Background:
- Nucleoside modifications are crucial in various biological processes.
- Enzymatic deacetylation offers a green chemistry approach for modifying nucleosides.
- Previous studies focused on lipase-catalyzed reactions, leaving microbial whole-cell biocatalysis unexplored for this purpose.
Purpose of the Study:
- To investigate the enzymatic hydrolysis of acetylated nucleosides using microbial whole cells for the first time.
- To compare the deacetylation profiles of different microorganisms.
- To explore the regioselectivity of the deacetylation process.
Main Methods:
- Screening of various microbial whole-cell biocatalysts for deacetylation activity.
- Enzymatic hydrolysis of different acetylated nucleoside substrates.
- Analysis of deacetylation products using chromatographic and spectroscopic techniques.
Main Results:
- Demonstrated the feasibility of using microbial whole cells for nucleoside deacetylation.
- Observed diverse deacetylation profiles among tested microorganisms, unlike the common profile seen with Candida antarctica B lipase.
- Achieved 5'-selective deprotection or mixtures of mono O-acetylated products, contingent on the specific substrate and biocatalyst employed.
Conclusions:
- Microbial whole cells represent a novel biocatalytic system for acetylated nucleoside hydrolysis.
- The regioselectivity of deacetylation is highly dependent on the microbial strain and substrate.
- This study opens new avenues for selective nucleoside modification using microbial biocatalysis.
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