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[Development of an immunochromayography assay method for the detection of Yersinia pestis]
Hong Zhu1, Chun-hua Zhang, Hua Tan
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Objective:
To develop a method of immunochromatography assay (ICA) with sensitive, specific, rapid, simple and suitable for the detection of Yersinia pestis antigen at the local laboratories.
Methods:
Colloidal gold labeled with the anti-F1 antibody of Yersinia pestis, was connnected with the anti-F1 antibody of Yersinia pestis to pyroxylin membrane and assembled them to the dipstick of ICA.
Results:
Results showed that the rates of sensitivity for F1 antigen and Yersinia pestis were 1 ng/ml and 1.56 x 10(5) CFU/ml respectively. However, Yersinia pseudotuberculosis et al could not be detected by dipstick of ICA.
Conclusion:
The method of ICA appeared to be consistent to those of r-IHA with better specificity and sensitivity but was simple and rapid for the detection of Yersinia pestis and F1 antigen.
