Microbial contamination of endodontic files received from the manufacturer

Todd P Roth1, Scott I Whitney, Stephen G Walker

  • 1Division of Endodontics, School of Dental Medicine, Stony Brook University, New York, USA. toddroth@hotmail.com

Insights

New endodontic files are often not sterile. Sterilizing them with sodium hypochlorite before use is recommended, and manufacturers should indicate sterility status on packaging.

Area of Science:

  • Microbiology
  • Dental Materials Science
  • Infection Control

Background:

  • New endodontic files may harbor microorganisms.
  • Ensuring instrument sterility is crucial for preventing iatrogenic infections.

Purpose of the Study:

  • To assess the microbial contamination of new endodontic files.
  • To evaluate the efficacy of a chairside sterilization method for endodontic files.

Main Methods:

  • Tested 150 new endodontic files (hand and rotary) from five manufacturers for microbial contamination.
  • Intentionally contaminated autoclaved files with recovered bacteria.
  • Evaluated sterilization using 5.25% sodium hypochlorite immersion for five minutes.

Main Results:

  • 13% of tested endodontic files showed positive microbial cultures.
  • Five-minute immersion in 5.25% sodium hypochlorite effectively sterilized contaminated files.

Conclusions:

  • Endodontic files require sterilization prior to clinical application.
  • Manufacturers should clearly label the sterility of endodontic files.

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