Related Experiment Video
Updated: Aug 7, 2026

Scanning Electron Microscopic Evaluation of Surface Defects of Remover Retreatment File After Single and Multiple Uses
Published on: October 11, 2024
Microbial contamination of endodontic files received from the manufacturer
Todd P Roth1, Scott I Whitney, Stephen G Walker
1Division of Endodontics, School of Dental Medicine, Stony Brook University, New York, USA. toddroth@hotmail.com
Abstract:
This study was conducted to test the sterility of new unused endodontic files received from manufacturers. Fifteen types of hand and rotary files from five manufacturers were tested. Positive microbial cultures were obtained from 13% of the 150 files tested. Autoclaved files were intentionally contaminated with bacterial species recovered from the positive cultures to evaluate a chairside sterilization method. Immersion of contaminated files in 5.25% sodium hypochlorite for five minutes sterilized the files. The results of this study indicate that endodontic files should be sterilized before clinical use. It is also suggested that manufacturers list the sterility state of their endodontic files on their packaging.
Insights
New endodontic files are often not sterile. Sterilizing them with sodium hypochlorite before use is recommended, and manufacturers should indicate sterility status on packaging.
Area of Science:
- Microbiology
- Dental Materials Science
- Infection Control
Background:
- New endodontic files may harbor microorganisms.
- Ensuring instrument sterility is crucial for preventing iatrogenic infections.
Purpose of the Study:
- To assess the microbial contamination of new endodontic files.
- To evaluate the efficacy of a chairside sterilization method for endodontic files.
Main Methods:
- Tested 150 new endodontic files (hand and rotary) from five manufacturers for microbial contamination.
- Intentionally contaminated autoclaved files with recovered bacteria.
- Evaluated sterilization using 5.25% sodium hypochlorite immersion for five minutes.
Main Results:
- 13% of tested endodontic files showed positive microbial cultures.
- Five-minute immersion in 5.25% sodium hypochlorite effectively sterilized contaminated files.
Conclusions:
- Endodontic files require sterilization prior to clinical application.
- Manufacturers should clearly label the sterility of endodontic files.
Related Concept Videos
Sources of Food Contamination
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Good Manufacturing Practices
