Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell-cell interactions on solid matrices.

Nancy A Louis1, Dionne Daniels, Sean P Colgan

  • 1Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 27, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic host-microbe crosstalk in stabilization of epithelial HIF.

Microbiome·2026
Same author

Impact of Neutrophils on the Tissue Microenvironment During Intestinal Inflammation.

Journal of inflammation research·2026
Same author

Eosinophil-derived COX-2 protects against experimental colitis through the PGE<sub>2</sub>-IL-22 axis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Neutrophil-derived reactive oxygen species and bystander tissue damage in inflammatory bowel disease.

Free radical biology & medicine·2026
Same author

Metabolite mimicry identifies butyrate analogs with select protective functions in the intestinal mucosa.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Mitochondrial ATP production promotes T cell differentiation and function by regulating chromatin accessibility.

bioRxiv : the preprint server for biology·2026
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

New in vitro models use membrane permeable supports to mimic in vivo cell organization, enabling the study of cell-cell contact and mediator discovery for physiological processes.

Area of Science:

  • * Cellular and Molecular Biology
  • * Biomedical Engineering
  • * Physiology

Background:

  • * In vitro models often use randomly organized cells, failing to replicate in vivo physiological complexity.
  • * Accurate in vivo responses necessitate organized, three-dimensional cell architecture and defined cellular interfaces.
  • * Previous models focused on substrate-grown cells for studying cell-cell contact.

Purpose of the Study:

  • * To review the application of membrane permeable supports for in vitro cell culture models.
  • * To highlight the utility of these models in studying physiologically positioned cell types.
  • * To discuss their role in discovering mediators of physiological and pathophysiological processes.

Main Methods:

  • * Utilizing membrane permeable supports to culture multiple cell types in defined spatial arrangements.

Related Experiment Videos

  • * Incorporating nonadherent cells, such as leukocytes, in co-culture systems.
  • * Investigating functional responses based on cell-cell interactions within these structured models.

    • Main Results:

    • * These models successfully replicate organized cellular architecture and interfaces.
    • * They facilitate the study of functional responses in physiologically relevant cell arrangements.
    • * The models have proven effective in identifying both cell-bound and soluble mediators.

    Conclusions:

    • * Membrane permeable supports offer a robust platform for advanced in vitro cell modeling.
    • * These models provide superior physiological relevance compared to traditional random cell cultures.
    • * They are instrumental in advancing the understanding of cell-mediated physiological and pathophysiological events.