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Methods to assess tissue permeability.

Juan C Ibla1, Joseph Khoury

  • 1Department of Anesthesiology, Perioperative, and Pain Medicine, Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women's Hospital, Children's Hospital Boston, Harvard Medical School, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 27, 2006
PubMed
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This chapter details methods for measuring organ permeability, crucial for understanding endothelial cell barrier function and vascular leak in disease.

Area of Science:

  • Physiology
  • Cell Biology
  • Vascular Biology

Background:

  • Organ function relies on permeability barriers, primarily the endothelial cell lining of blood vessels.
  • Disruption of this barrier leads to increased vascular permeability and leakage, linked to various systemic diseases.

Purpose of the Study:

  • To describe systematic methods for measuring the permeability characteristics of multiple organs.
  • To highlight the importance of in vivo data and mammalian models for understanding physiological significance.

Main Methods:

  • Systematic measurement of organ permeability characteristics.
  • Utilizing in vivo data to complement molecular findings.
  • Employing mammalian models to study interactions between multiple cell types and tissues.

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Main Results:

  • Established methods for assessing organ permeability.
  • Demonstrated the value of in vivo data in understanding barrier function.
  • Validated the physiological relevance of endothelial barrier mechanisms in complex tissues.

Conclusions:

  • Accurate measurement of organ permeability is vital for understanding endothelial barrier function.
  • A multidisciplinary approach integrating in vivo and molecular data is essential.
  • Mammalian models provide critical insights into the physiological roles of barrier mechanisms.