Related Experiment Video
Updated: Aug 7, 2026

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors
Published on: July 17, 2020
MAP kinases and heat shock-induced hormesis in human fibroblasts during serial passaging in vitro
Elise R Nielsen1, Yvonne E G Eskildsen-Helmond, Suresh I S Rattan
1Laboratory of Cellular Ageing, Danish Centre for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, Denmark. elisernielsen@hotmail.com
Abstract:
Adult human skin fibroblasts were exposed repeatedly to 41 degrees C or 42 degrees C heat shock (HS) for 1 h twice a week during serial passaging throughout their replicative life span. On the basis of longevity curves, cell size, and morphology, we observed that repeated mild heat shock (RMHS) at 41, degrees C had strong anti-aging hormetic effects, including 20% extension of cellular longevity. The basal levels of the MAP kinases JNK1, JNK2, and p38 increased during serial passaging, while that of ERK2 decreased. RMHS further exaggerated these effects, which suggests that age-related changes in MAP kinases may be an adaptive response for better cell survival.
Related Concept Videos
MAPK Signaling Cascades
Mitogens and the Cell Cycle
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
PI3K/mTOR/AKT Signaling Pathway
Cellular Adaptation II: Hypertrophy

