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A microfluidic device for continuous, real time blood plasma separation.

Sung Yang1, Akif Undar, Jeffrey D Zahn

  • 1205, Hallowell, Department of Bioengineering, The Pennsylvania State University, University Park, PA, USA. sxy154@psu.edu

Lab on a Chip
|June 29, 2006
PubMed
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This study introduces a novel microfluidic device for continuous, real-time blood plasma separation. The device efficiently separates plasma from red blood cells using the Zweifach-Fung effect, achieving nearly 100% plasma selectivity without clogging or cell damage.

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Biotechnology

Background:

  • Continuous, real-time blood plasma separation is crucial for various lab-on-a-chip applications.
  • Existing methods can be complex or lead to cell damage.
  • The Zweifach-Fung effect offers a potential mechanism for efficient cell-free plasma extraction.

Purpose of the Study:

  • To develop and demonstrate a microfluidic device for continuous, real-time blood plasma separation.
  • To validate the device's performance using simple microchannels and the Zweifach-Fung effect.
  • To assess the device's efficiency, selectivity, and biocompatibility for potential lab-on-a-chip integration.

Main Methods:

  • Design of a microfluidic network using electrical circuit analogy, analytical, and numerical studies.

Related Experiment Videos

  • Experimental demonstration using a device with a blood inlet, bifurcating outlets for plasma and concentrated cells.
  • Testing with defibrinated sheep blood at varying hematocrit levels (up to 45%).

    • Main Results:

    • Continuous, clog-free, and hemolysis-free operation for 30 minutes demonstrated.
    • Nearly 100% plasma selectivity achieved across all tested inlet hematocrit levels.
    • Plasma separation volume percentage ranged from 15% to 25% with increasing hematocrit.

    Conclusions:

    • The developed microfluidic device enables highly efficient, continuous, real-time, cell-free blood plasma separation.
    • The simple design and reliable performance make it suitable for lab-on-a-chip applications.
    • The Zweifach-Fung effect is effectively utilized for precise plasma separation from whole blood.