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Published on: April 16, 2018
Reactive azo dye reduction by Shewanella strain J18 143
Carolyn I Pearce1, Robert Christie, Christopher Boothman
1Williamson Research Centre for Molecular Environmental Science and School of Earth, Atmospheric and Environmental Sciences, The University of Manchester, Manchester M13 9PL, UK.
Biotechnology and Bioengineering
|June 29, 2006
Summary
A Shewanella bacterium effectively decolorizes reactive azo dyes like Remazol Black B. This microbial reduction process, particularly under anaerobic conditions, shows significant potential for treating textile wastewater contaminants.
Area of Science:
- Microbiology
- Environmental Science
- Biotechnology
Background:
- Textile wastewater often contains persistent reactive azo dyes.
- Efficient and environmentally friendly dye decolorization methods are needed.
Purpose of the Study:
- To isolate and identify a bacterial strain capable of decolorizing azo dyes.
- To investigate the mechanism and efficiency of dye reduction by the identified bacterium.
Main Methods:
- Phylogenetic analysis using 16S rRNA gene sequencing.
- Analysis of dye decolorization end products using capillary electrophoresis, UV-visible spectrophotometry, and LC-MS.
- Assessment of various electron donors and process variables.
Main Results:
- A Shewanella species (strain J18 143) was identified as an effective azo dye reducer.
- Decolorization occurs via microbially mediated reduction of the dye chromophore.
- Anaerobic conditions enhanced dye reduction efficiency by 30-fold compared to aerated cultures.
- Formate was identified as the optimal electron donor.
Conclusions:
- Shewanella strain J18 143 demonstrates high efficiency in reducing azo dyes.
- The bacterium offers advantages as a biocatalyst for azo dye treatment in industrial wastewater.
