Related Experiment Video
Updated: Aug 7, 2026

Visualization of Organelles In Situ by Cryo-STEM Tomography
Published on: June 23, 2023
Observations of unstained biological specimens using a low-energy, high-resolution STEM
Akio Takaoka1, Toshiaki Hasegawa
1Research Center for Ultra-High Voltage Electron Microscopy, Osaka University Mihoga-oka 7-1, Ibaraki, Osaka 567-0047, Japan. takaoka@uhvem.osaka-u.ac.jp
Abstract:
Low-energy, high-resolution scanning transmission electron microscopy (STEM) is introduced as a convenient method for observing unstained biological specimens. By reducing the electron energy, the cross section for light elements becomes comparable to that of conventional electron microscopy observations. The STEM mode exhibited the advantage that the induced energy loss and charge build-up in the sample affected the image to a lesser extent than in the TEM or SEM mode. Furthermore, the efficiency of an STEM detector is high, and the total radiation damage can be reduced if thermal damage due to localized heating at a slow scan operation can be overcome. We applied this method for observations of biological samples that were in the form of thin slices, fine fibers and small particles. When the supporting film for samples is absent, the resolution and the contrast of STEM images can be maintained similar to SEM and TEM images, respectively.
Related Concept Videos
Overview of Electron Microscopy
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Overview of Microscopy Techniques
Scanning Electron Microscopy
Fundamental Principles
Accelerated...
Transmission Electron Microscopy
Super-resolution Fluorescence Microscopy

