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Related Experiment Videos

Hypothermia modulates circadian clock gene expression in lizard peripheral tissues.

Daniela Vallone1, Elena Frigato, Cristiano Vernesi

  • 1Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35, Tübingen D-72076, Germany.

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
|July 1, 2006
PubMed
Summary

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Researchers identified key circadian clock genes in the ruin lizard, Podarcis sicula. Low temperatures significantly alter clock gene expression in peripheral tissues, suggesting a conserved vertebrate response to cold and hypothermia.

Area of Science:

  • Chronobiology
  • Molecular Biology
  • Vertebrate Physiology

Background:

  • Circadian clock mechanisms responding to temperature are not well understood.
  • The ruin lizard (Podarcis sicula) is a suitable ectotherm model for studying temperature effects on circadian rhythms.
  • Lack of molecular tools hindered previous research in this model organism.

Purpose of the Study:

  • To clone and characterize lizard circadian clock gene homologs.
  • To investigate the impact of temperature on the expression of these clock genes.
  • To understand the molecular basis of circadian adaptation to temperature variations in vertebrates.

Main Methods:

  • Cloning of Period2, Cryptochrome1, and Clock gene homologs in Podarcis sicula.
  • Analysis of gene expression in various tissues under different temperature conditions (29°C and 6°C).

Related Experiment Videos

  • Comparison of expression profiles under light-dark and constant darkness cycles.

    • Main Results:

    • Three lizard clock gene homologs, phylogenetically related to avian genes, were successfully cloned.
    • These genes exhibit rhythmic expression in multiple tissues at 29°C, similar to mammalian and avian counterparts.
    • At low temperatures (6°C), peripheral clock gene expression cycling is attenuated, with elevated basal levels.

    Conclusions:

    • The cloned lizard clock genes are functional components of the circadian system.
    • Low temperatures attenuate peripheral circadian clock gene expression, indicating a conserved vertebrate response.
    • Findings provide insights into the compatibility of circadian clock function with hypothermia.