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Updated: Aug 7, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Published on: May 27, 2016
Electrostatic properties of promoter recognized by E. coli RNA polymerase Esigma70
Anatoly A Sorokin1, Alexandr A Osypov, Timur R Dzhelyadin
1Laboratory of Mechanisms of the Cell Genom Functioning, Institute of Cell Biophysics RAS, Pushchino, 142290, Russia. lptolikr@icb.psn.ru
Abstract:
A comparative analysis of electrostatic patterns for 359 sigma70-specific promoters and 359 nonpromoter regions on electrostatic map of Escherichia coli genome was carried out. It was found that DNA is not a uniformly charged molecule. There are some local inhomogeneities in its electrostatic profile which correlate with promoter sequences. Electrostatic patterns of promoter DNAs can be specified due to the presence of some distinctive motifs which differ for different promoter groups and may be involved as signal elements in differential recognition of various promoters by the enzyme. Some specific electrostatic elements which are responsible for modulating promoter activities due to ADP-ribosylation of RNA polymerase alpha-subunit were found in far upstream regions of T4 phage early promoters and E. coli ribosomal promoters.
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