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Intrinsic properties of so-called dormant probiotic bacteria, determined by flow cytometric viability assays
Sampo J Lahtinen1, Arthur C Ouwehand, Johanna P Reinikainen
1Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland. sajola@utu.fi
Applied and Environmental Microbiology
|July 6, 2006
Abstract:
Plate counting and four culture-independent flow cytometric assays were used to determine the viability and intrinsic properties of three probiotic strains during storage. The strains showed reduction in plate counts but were able to maintain esterase activity, intact cytoplasmic membrane, and pH gradient. The apparently uncultivable probiotic cells were active and stress resistant.
