Related Experiment Video

Updated: Aug 7, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Activated Notch1 alters differentiation of embryonic stem cells into mesodermal cell lineages at multiple stages of

Timm Schroeder1, Franziska Meier-Stiegen, Ralf Schwanbeck

  • 1Institute for Clinical Molecular Biology and Tumor Genetics, GSF - National Research Center for Environment and Health, Marchioninistr. 25, 81377 München, Germany. timm.schroeder@gsf.de

Mechanisms of Development
|July 11, 2006
PubMed

Insights

Notch signaling regulates embryonic development. Activating Notch1 in stem cells alters mesodermal cell generation, impacting cardiac, blood vessel, and hematopoietic cell development.

Area of Science:

  • Developmental Biology
  • Cell Signaling
  • Stem Cell Biology

Background:

  • Notch transmembrane receptors are crucial for regulating diverse developmental cell fates.
  • Understanding Notch signaling's role in mesodermal lineage development is essential for regenerative medicine and developmental studies.

Purpose of the Study:

  • To investigate the role of Notch signaling in the development of mesodermal cell types.
  • To determine how timed activation of Notch1 signaling affects differentiation of embryonic stem cells (ESC) into various mesodermal lineages.

Main Methods:

  • Utilized a tamoxifen-inducible, activated form of Notch1 expressed in ESC.
  • Employed the OP9 stroma co-culture system for differentiation into mesodermal progenitor, endothelial, mural, cardiac muscle, and hematopoietic cells.
  • Administered tamoxifen at various differentiation stages to control Notch activation timing.

Main Results:

  • Activated Notch signaling inhibited differentiation of ESC into Flk1(+) mesodermal cells.
  • Activation in mesodermal cells suppressed cardiac muscle, endothelial, and hematopoietic cell generation, promoting mural cell production.
  • Notch activation in hematopoietic cells reduced colony formation and hematopoietic maintenance.

Conclusions:

  • Notch signaling plays a critical regulatory role in mesodermal development.
  • It influences cardiomyogenesis and the balance between endothelial and mural cell generation in blood vessels.
  • Notch signaling is important for proper hematopoietic development.

More Related Videos

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay06:49

Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

Published on: January 12, 2022

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...
Gastrulation01:56

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells: