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Decellularized umbilical artery treated with thin polyelectrolyte multilayer films: potential use in vascular
H Kerdjoudj1, C Boura, L Marchal
1Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA-UMR 7563, IFR III Faculté de Médecine, Université Henry Poincaré Nancy 1, 54505 Vandoeuvre-lès-Nancy, France. halima.kerdjoudj@medecine.uhp-nancy.fr
Bio-Medical Materials and Engineering
|July 11, 2006
Summary
Decellularized human umbilical arteries coated with polyelectrolyte multilayer films show potential as vascular scaffolds. This surface modification improves the extracellular matrix for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Vascular Surgery
Background:
- Decellularized allograft tissues serve as extracellular matrix scaffolds for engineered vascular substitutes.
- Improving thromboresistance of vascular scaffolds requires pre-coating the luminal surface.
Purpose of the Study:
- To develop an alternative vascular scaffold using decellularized human umbilical arteries.
- To apply a poly(allylamine hydrochloride)/poly(styrenesulfonate) (PAH/PSS) polyelectrolyte multilayer film for surface modification.
Main Methods:
- Decellularization of human umbilical arteries.
- Surface modification via alternate adsorption of PAH/PSS polyelectrolytes.
- Scanning electron microscopy (SEM) and confocal microscopy for surface analysis.
Main Results:
- Luminal surfaces were completely devoid of endothelial cells (ECs) after trypsin treatment.
- The PAH/PSS multilayer film uniformly coated the internal surface of the vessels.
- Confocal microscopy confirmed successful deposition and retention of the fluorescently labeled multilayer film.
Conclusions:
- Decellularized cryopreserved arteries are a promising scaffold for vascular tissue engineering.
- Polyelectrolyte multilayer films can be tailored to promote or inhibit cell adhesion based on the terminal layer.
