Related Experiment Video
Updated: Aug 7, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
Published on: November 1, 2024
Analysis of interdomain dynamics in a two-domain protein using residual dipolar couplings together with 15N
Yaroslav Ryabov1, David Fushman
1Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, MD 20742, USA.
Abstract:
In this paper, we propose the idea that simultaneous analysis of NMR relaxation data and residual dipolar couplings (RDCs) can provide information about interdomain dynamics in a multidomain protein, which cannot be derived from each data set separately. Specifically, such an approach can be useful when the interdomain motions occur on a timescale comparable to or slower than the overall tumbling in solution. We analyze residual dipolar couplings together with 15N relaxation data for Lys48-linked di-ubiquitin (Ub2), in which interdomain dynamics are described as interconversion between two distinct conformational states of the protein. Our results show that 15N relaxation and residual dipolar coupling data can be used as two complementary experimental data sets for consistent characterization of interdomain conformations and dynamics in this dual-domain protein.
Related Concept Videos
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
2D NMR: Overview of Homonuclear Correlation Techniques
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Overview of Heteronuclear Correlation Techniques
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
Conservation of Protein Domains
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...

