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Updated: Aug 7, 2026
Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
Published on: November 4, 2021
Micropatterning with aerosols: application for biomaterials
Louis Gagné1, Gerardo Rivera, Gaetan Laroche
1Unité de biotechnologie et de bioingénierie, Centre de recherche du CHUQ, Hôpital Saint-François d'Assise, and Département de génie des mines, de la métallurgie et des matériaux, Faculté des sciences et de génie, Université Laval, Qué., Canada.
Abstract:
Adhesion and proliferation behaviors of bovine aortic endothelial cells (BAECs) were investigated on surfaces micropatterned with peptides using a novel approach. This micropatterning technique allows modification of macroscopic three-dimensional (3D) biomaterials surfaces and exploits the semi-random properties of aerosols and the principles of liquid atomization. The possibility to control cell behaviors on polytetrafluoroethylene (PTFE) surfaces tailored with this micropatterning approach was evaluated. CGRGDS and CWQPPRARI peptides were selected for their adhesive, migration and spreading properties. Culture of BAECs on patterned PTFE showed the possibility of modulating cell behaviors. The study showed that CGRGDS spots with a diameter of 10+/-2 microm over a background of CWQPPRARI peptides was the most effective combination to enhance endothelialization of PTFE. This micropatterning technique is innovative, easily adaptable, simple, and rapid for covering large 3D areas.
